MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655 : alkaline phosphatase Inferred from experiment : phoA
acid phosphatase / phosphotransferase : aphA
broad specificity 5'(3')-nucleotidase and polyphosphatase : umpG
pyrimidine nucleotidase : yjjG
UMP phosphatase : umpH
5'-ribonucleotidase : ushA
Homo sapiens : 5'-nucleotidase Inferred from experiment : NT5E
cytosolic 5'-nucleotidase 1A Inferred from experiment : NT5C1A
Solanum tuberosum : 5'-nucleotidase Inferred from experiment

In Pathway: adenosine nucleotides degradation II

Note that this reaction equation differs from the official Enzyme Commission reaction equation for this EC number, which can be found here .

Reaction Locations: periplasmic space (sensu Gram-negative Bacteria), cytosol

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 5′-nucleotidase

Enzyme Commission Synonyms: uridine 5'-nucleotidase, 5'-adenylic phosphatase, adenosine 5'-phosphatase, AMP phosphatase, adenosine monophosphatase, 5'-mononucleotidase, AMPase, UMPase, snake venom 5'-nucleotidase, thimidine monophosphate nucleotidase, 5'-AMPase, 5'-AMP nucleotidase, AMP phosphohydrolase, IMP 5'-nucleotidase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 6.127014 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Wide specificity for 5-nucleotides.

Citations: [Gulland38, Heppel51, Segal60]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reaction of [a ribonucleoside 5'-monophosphate + H2O → a ribonucleoside + phosphate] (
i1: AMP + H2O → adenosine + phosphate (

Unification Links: KEGG:R00183 , Rhea:29375

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC: , UniProt:RELATED-TO:O29385 , UniProt:RELATED-TO:O34313 , UniProt:RELATED-TO:O83142 , UniProt:RELATED-TO:P06196 , UniProt:RELATED-TO:P07024 , UniProt:RELATED-TO:P21588 , UniProt:RELATED-TO:P21589 , UniProt:RELATED-TO:P22848 , UniProt:RELATED-TO:P29240 , UniProt:RELATED-TO:P44569 , UniProt:RELATED-TO:P49902 , UniProt:RELATED-TO:Q9KM44 , UniProt:RELATED-TO:Q05927


Gulland38: Gulland JM, Jackson EM (1938). "5-Nucleotidase." Biochem J 32(3);597-601. PMID: 16746659

Heppel51: Heppel LA, Hilmore RJ (1951). "Purification and properties of 5-nucleotidase." J Biol Chem 188(2);665-76. PMID: 14824154

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Segal60: Segal HL, Brenner BM (1960). "5'-Nucleotidase of rat liver microsomes." J Biol Chem 235;471-4. PMID: 14444527

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Jul 28, 2015, BIOCYC13B.