Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: allantoate amidohydrolase

Gene: AAH Accession Number: AT4G20070 (MetaCyc)

Synonyms: At4g20070, AtAAH

Species: Arabidopsis thaliana col

Subunit composition of allantoate amidohydrolase = [AAH]2
         allantoate amidohydrolase monomer = AAH

Summary:
The cDNA encoding allantoate amidohydrolase was isolated from Arabidopsis thaliana col. The enzyme was initially reported to catalyzes the conversion of allantoate to yield S-ureidoglycolate via a (-)-ureidoglycine [Todd06], but a later study showed that the enzyme catalyzes only the first part - conversion of allantoate to a (-)-ureidoglycine [Werner10]

The function of the gene was confirmed by complementation of Saccharomyces cerevisiae DAL2 mutants, blocked in allantoate degradation. Mutants with T-DNA insertions in AAH, the gene encoding the enzyme, showed normal growth on standard medium, but growth was completely blocked when the sole N source was allantoin. The enzyme was expressed in all tissues indicating a vital role in metabolism. It was proposed that the enzyme is involved in the purine salvage pathway through ureide biosynthesis and degradation [Todd06].

The gene was subsequently cloned, expressed in planta in the form of several StrepII-tagged variants, and highly purified from leaf extracts. The enzyme requires Mn2+ and exists as a homodimer [Werner08].

Molecular Weight of Polypeptide: 56.524 kD (from nucleotide sequence)

Unification Links: Entrez:NP_193740 , PhylomeDB:O49434 , Pride:O49434 , Protein Model Portal:O49434 , SMR:O49434 , String:3702.AT4G20070.1-P , TAIR:At4g20070 , UniProt:O49434

Relationship Links: InterPro:IN-FAMILY:IPR001261 , InterPro:IN-FAMILY:IPR002933 , InterPro:IN-FAMILY:IPR010158 , InterPro:IN-FAMILY:IPR011650 , Pfam:IN-FAMILY:PF01546 , Pfam:IN-FAMILY:PF07687 , Prosite:IN-FAMILY:PS00758 , Prosite:IN-FAMILY:PS00759

Gene-Reaction Schematic: ?

Credits:
Created 10-May-2006 by Foerster H , TAIR
Revised 07-Nov-2007 by Caspi R , SRI International


Enzymatic reaction of: allantoate amidohydrolase

EC Number: 3.5.3.9

allantoate + 2 H+ + H2O <=> S-ureidoglycine + ammonium + CO2

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of allantoin degradation in plants , allantoin degradation to glyoxylate II , allantoin degradation to ureidoglycolate II (ammonia producing)

Citations: [Todd06]

Cofactors or Prosthetic Groups: Mn2+ [Werner08]


References

Todd06: Todd CD, Polacco JC (2006). "AtAAH encodes a protein with allantoate amidohydrolase activity from Arabidopsis thaliana." Planta 223(5);1108-13. PMID: 16496096

Werner08: Werner AK, Sparkes IA, Romeis T, Witte CP (2008). "Identification, biochemical characterization, and subcellular localization of allantoate amidohydrolases from Arabidopsis and soybean." Plant Physiol 146(2);418-30. PMID: 18065556

Werner10: Werner AK, Romeis T, Witte CP (2010). "Ureide catabolism in Arabidopsis thaliana and Escherichia coli." Nat Chem Biol 6(1);19-21. PMID: 19935661


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14A.